ThesisOpen AccessMetadata saja
2025•Skripsi, Thesis, Disertasi, dan Tugas Akhir
Kersen leaves contain phenolic compounds, flavonoids, tannins, alkaloids, saponins, and steroids. This study aims to determine the antibacterial activity of ethanol extract of leaves against Escherichia coli bacteria and to determine the potential antibacterial activity compared to gentamicin. Antibacterial activity testing was conducted using the well diffusion method with 7 treatment groups, namely 25%, 50%, 75%, and 100% concentrations of ethanol extract from kersen leaves, gentamicin 10μg/20μl as the positive control, water for injection as the negative control, and 96% ethanol as the solvent control. Each treatment was inoculated with bacteria, then dripped with ethanol extract of kersen leaves, incubated at 37°C for 24 hours, and the data obtained in the form of inhibition zones were measured and analyzed for sensitivity based on CLSI with gentamicin as a comparison. Antibacterial activity showed the diameter of the inhibition zone on E. coli bacteria at concentrations of 25%; 50%; 75% and 100% of kersen leaf extract measuring 13.66±0.16mm; 14.58±0.22mm; 15.16±0.15mm and 16.46±0.15mm, with intermediate categories at concentrations of 25% and 50% and sensitive at concentrations of 75% and 100%. Gentamicin showed an inhibition zone diameter of 17.66±0.20mm with a sensitive category according to CLSI. The results of the inhibition zone indicated a significant difference between the extract treatment groups and the positive control gentamicin (sig0.05). Based on the research results, it can be concluded that the ethanol extract of kersen leaves has antibacterial activity against E. coli bacteria, but its potency is not comparable to gentamicin.
ThesisOpen AccessMetadata saja
2024•Skripsi, Thesis, Disertasi, dan Tugas Akhir
The cherry plant has great potential to be utilized because the leaves of this plant contain chemical properties that are useful for health. Cherry leaves have several chemical contents such as alkaloids, flavonoids, tannins, saponins and steroids. The chemical content in plants can be obtained through an extraction process. Differences in solvent concentration in the extraction process can affect the yield and chemical content of the extract. This research aims to determine the solvent concentration on the yield and chemical content of cherry leaves. This type of research uses a post test only design. Extraction of cherry leaves using 70% and 96% ethanol solvent. Extraction was carried out using the maceration method for 3x24 hours. The extract obtained is evaporated by air, until the extract is thick. The results of the thick extract were calculated for yield and phytochemical screening. The yield values obtained were subjected to a normality test using Shapiro-Wilk, while the homogeneity test was carried out using the Levene's Test. Data that is normally and homogeneously distributed is followed by the Independent Sample T-Test. If the data is not normally distributed then analysis is carried out using Mann-Whitney. The results of the phytochemical screening in the form of chemical content of 70% and 96% ethanol cherry leaf extract are presented descriptively.
ThesisOpen AccessMetadata saja
2023•Skripsi, Thesis, Disertasi, dan Tugas Akhir
Daun kersen berkhasiat sebagai penyembuhan asam urat, antiseptik, antitumor dan antiinflamasi. Daun kersen mengandung beberapa senyawa bioaktif seperti saponin, flavonoid dan tanin. Terdapat perbedaan pelarut terhadap rendemen dan kandungan kimia. Tujuan penelitian ini untuk mengetahui pengaruh perbedaan jenis pelarut terhadap nilai rendemen dan kandungan kimia ekstrak daun kersen.
Metode yang digunakan pada penelitian ini yaitu eksperimental post-test only design. Daun kersen dikeringkan menggunakan pengeringan oven pada suhu 50˚C selama 10 jam dan dilakukan maserasi selama 3x24 jam, menggunakan pelarut etanol 96% dan n-heksana. Ekstrak kental yang diperoleh dihitung rendemen dan diuji skrining fitokimia. Nilai rendemen dianalisis secara statistik dengan Independent Sampel T-Test. Hasil skrining fitokimia akan dibandingkan dengan penelitian sebelumnya.
ThesisOpen AccessMetadata saja
2023•Skripsi, Thesis, Disertasi, dan Tugas Akhir
Daun kersen memiliki manfaat sebagai antibakteri, antioksidan, antidiabetes dan antiinflamasi. Pengeringan simplisia daun kersen dilakukan untuk mengurangi kadar air dalam simplisia. Perbedaan metode pengeringan simplisia dapat mempengaruhi kadar air simplisia. Tujuan penelitian ini untuk mengetahui pengaruh perbedaan metode pengeringan terhadap kadar air simplisia daun kersen. Metode yang digunakan pada penelitian ini, yaitu eksperimental Postest Only Design. Daun kersen dikeringkan menggunakan 2 metode, yaitu pengeringan sinar matahari dan oven kemudian dilakukan penetapan kadar air menggunakan metode gravimetri. Data kadar air yang diperoleh dibandingkan dengan parameter quality control pengeringan berdasarkan pedoman umum pascapanen tanaman obat kemudian pengujian dilanjutkan dengan uji Independet Sampel T test. Hasil rata-rata nilai kadar air simplisia daun kersen dengan metode pengeringan sinar matahari sebesar 8,07±0,08% dan oven sebesar 7,35±0,05%. Hasil analisis statistik menunjukkan bahwa terdapat perbedaan yang bermakna pada kadar air dan setiap metode pengeringan. Hasil penelitian menunjukan bahwa perbedaan metode pengeringan dapat berpengaruh terhadap kadar air simplisia daun kersen.
