ThesisOpen AccessMetadata saja
2025•Skripsi, Thesis, Disertasi, dan Tugas Akhir
Limbah biji pepaya memiliki kandungan kimia berupa karpain dan belum dimanfaatkan secara optimal. Kandungan kimia tersebut bersifat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui manfaat limbah biji pepaya sebagai sabun cuci piring dengan aktivitas antibakteri Staphylococcus aureus. Metode penelitian menggunakan posttest only control group design. Biji pepaya diekstraksi dengan pelarut alkohol 96% secara maserasi selama 72 jam. Hasil ekstrak dibuat sabun cuci piring. Pengujian dilakukan dengan metode difusi cakram menggunakan 4 kelompok, 3 kelompok perlakuan dan 1 kelompok kontrol yaitu sabun cuci piring ekstrak biji pepaya dengan konsentrasi 2,5%, 5%, 7,5% dan kontrol positif menggunakan sabun cuci piring cair (sunlight). Setiap perlakuan diinkubasi pada suhu 37 °C selama 24 jam. Data yang diperoleh dianalisis secara statistik dengan uji normalitas menggunakan Shapiro Wilk dan uji homogenitas menggunakan Lavene’s test. Data yang terdistribusi normal dan homogen, dilanjutkan uji One Way Anova dan dilakukan analisis Least Significant Difference. Data yang tidak terdistribusi normak dan tidak homogen diuji dengan Kruskal wallis dan dilanjutkan dengan Mann-Whitney.
ThesisOpen AccessMetadata saja
2025•Skripsi, Thesis, Disertasi, dan Tugas Akhir
Kersen leaves contain phenolic compounds, flavonoids, tannins, alkaloids, saponins, and steroids. This study aims to determine the antibacterial activity of ethanol extract of leaves against Escherichia coli bacteria and to determine the potential antibacterial activity compared to gentamicin. Antibacterial activity testing was conducted using the well diffusion method with 7 treatment groups, namely 25%, 50%, 75%, and 100% concentrations of ethanol extract from kersen leaves, gentamicin 10μg/20μl as the positive control, water for injection as the negative control, and 96% ethanol as the solvent control. Each treatment was inoculated with bacteria, then dripped with ethanol extract of kersen leaves, incubated at 37°C for 24 hours, and the data obtained in the form of inhibition zones were measured and analyzed for sensitivity based on CLSI with gentamicin as a comparison. Antibacterial activity showed the diameter of the inhibition zone on E. coli bacteria at concentrations of 25%; 50%; 75% and 100% of kersen leaf extract measuring 13.66±0.16mm; 14.58±0.22mm; 15.16±0.15mm and 16.46±0.15mm, with intermediate categories at concentrations of 25% and 50% and sensitive at concentrations of 75% and 100%. Gentamicin showed an inhibition zone diameter of 17.66±0.20mm with a sensitive category according to CLSI. The results of the inhibition zone indicated a significant difference between the extract treatment groups and the positive control gentamicin (sig0.05). Based on the research results, it can be concluded that the ethanol extract of kersen leaves has antibacterial activity against E. coli bacteria, but its potency is not comparable to gentamicin.
ThesisOpen AccessMetadata saja
2024•Skripsi, Thesis, Disertasi, dan Tugas Akhir
Kersen leaves has known to have antibacterial activity. This study aims to determine the antibacterial activity of kersen leaves ethanol extract against S. epidermidis bacteria and to determine the potential activity against S. epidermidis bacteria compared to gentamicin. Kersen leaves were extracted by maceration with 96% ethanol solvent for 24 hours. Tests were carried out using the well-diffusion method using 7 treatment groups, kersen leaves ethanol extract concentrations of 25%, 50%, 75%, 100% as test sample, gentamicin concentration of 10µg as a positive control, aqua pro injection as a negative control, and 96% ethanol as a solvent control. Each treatment was incubate at 35oC for 24 hours. The data obtained in the form of inhibition zones were and analyzed and measure for sensitivity based on CLSI. Antibacterial activity indicates the diameter of inhibition zone of bacteria S. epidermidis in concentration 25%, 50%, 75%, and 100% respectively of 11,46±0,15mm; 9,26±0,20mm; 7,80±0,10mm dan 6,30±0,20mm with the category of resistnace and gentamicin showed the diameter of inhibition zone was 12,06±0,11mm with a resistanc category. The conclusion of the study, kersen leaves extract has antibacterial activity against S. epidermidis bacteria, but its potency is not comparable to gentamicin in inhibiting the growth of S. epidermidis
ThesisOpen AccessMetadata saja
2023•Skripsi, Thesis, Disertasi, dan Tugas Akhir
INTISARI
Daun katuk memiliki kandungan kimia berupa polifenol, alkaloid, flavonoid, saponin, tanin, dan triterpernoid yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun katuk terhadap bakteri Staphylococcus epidermidis dan mengetahui potensi aktivitas ekstrak daun katuk sebagai antibakteri terhadap bakteri S. epidermidis dibandingkan dengan siprofloksasin.
Metode penelitian menggunakan post test only control group design. Pengujian dilakukan dengan metode difusi sumuran menggunakan 8 kelompok perlakuan, yaitu ekstrak daun katuk konsentrasi 20%, 40%, 60%, 80%, 100%, siprofloksasin konsentrasi 5µg sebagai kontrol positif, aqua pro injection sebagai kontol negative, dan etanol 96% sebagai kontrol pelarut. Diameter zona hambat yang dihasilkan merupakan data yang akan diukur dan dianalisa berdasarkan Clinical and Laboratory Standard Institute (CLSI). Data yang diperoleh kemudian dianalisis menggunakan SPSS. Pengujian normalitas dan homogenitas menggunakan Shapiro Wilk dan Levene’s test. Data yang terdistibusi normal dan homogen diuji dengan One Way Anova dan dilanjutkan dengan uji Least Significant Difference. Data yang tidak terdistribusi normal dan tidak homogen diuji dengan Kruskal wallis dan dilanjutkan dengan Mann-whitney
